Journal: Oncology Letters

Article Title: Increased soluble E‑cadherin of spheroid formation supplemented with fetal bovine serum in colorectal cancer cells

doi: 10.3892/ol.2023.13793

Figure Lengend Snippet: Cell viability changes after spheroid formation of colorectal cancer cells in different environments. (A) The SNU-C5 and SNU-C5/5-FUR cells were mock-treated with DMSO or treated with indicated doses of 5-FU for 3 days. The extent of cell viability was determined by MTT assay. Data are presented as the mean ± SD (n=3). *P<0.05, **P<0.01 and ***P<0.001 vs. DMSO; # P<0.05 and ## P<0.01 vs. sphere/GF. (B) The SNU-C5 and SNU-C5/5-FUR cells were mock-treated with DMSO or treated with indicated doses of 5-FU for indicated days. The extent of cell viability was determined by MTT assay using the aforementioned procedures. Data are presented as the mean ± SD (n=3). (C) Expression levels of cell cycle-related proteins in monolayer and spheroid formation cultures in SNU-C5 and SNU-C5/5-FUR cells were detected by immunoblotting. Immunoblotting analysis was performed for p21, β-catenin and GSK3β, while GAPDH was used for a loading control. Band density was analyzed by AzureSpot analysis software, and results are expressed as the mean ± SD (n=3). **P<0.01 and ***P<0.001 vs. monolayer; ## P<0.01 vs. sphere/FBS. 5-FU, 5-fluorouracil; FBS, fetal bovine serum; GF, growth factor.

Article Snippet: CREB (1:1,000; cat. no. CSB-PA005947HA01HU; Cusabio Technology, LLC), fibronectin (1:2,000; cat. no. CL54951AP; Cedarlane Laboratories), p21 (1:1,000; cat. no. 60214-1; Proteintech Group, Inc.) and p90RSK (Ab348; 1:1,000; cat. no. 79-554; Prosci, Inc.) were obtained from the corresponding listed company.

Techniques: MTT Assay, Expressing, Western Blot, Control, Software

Journal: Oncology Letters

Article Title: Increased soluble E‑cadherin of spheroid formation supplemented with fetal bovine serum in colorectal cancer cells

doi: 10.3892/ol.2023.13793

Figure Lengend Snippet: Densitometric results of western blotting on SNU-C5 and SNU-C5/5-FUR cells.

Article Snippet: CREB (1:1,000; cat. no. CSB-PA005947HA01HU; Cusabio Technology, LLC), fibronectin (1:2,000; cat. no. CL54951AP; Cedarlane Laboratories), p21 (1:1,000; cat. no. 60214-1; Proteintech Group, Inc.) and p90RSK (Ab348; 1:1,000; cat. no. 79-554; Prosci, Inc.) were obtained from the corresponding listed company.

Techniques: Western Blot, Marker

Journal: Frontiers in Behavioral Neuroscience

Article Title: Adiponectin Mediates the Protection of H 2 S Against Chronic Restraint Stress-Induced Cognitive Impairment via Attenuating Hippocampal Damage

doi: 10.3389/fnbeh.2021.623644

Figure Lengend Snippet: Effects of Anti-Acrp30 on NaHS-mitigated hippocampal oxidative stress in the CRS-treated rats. Rats were co-treated with NaHS (100 μmol/kg/day, i.p.) and CRS (6 h/day) for 3 weeks, and then co-administrated with Anti-Acrp30 (1 μg/day, i.c.v.) for further 1 week. (A) The content of hippocampal MDA was measured by malondialdehyde (MDA) enzyme-linked immunosorbent assay (ELISA) kit. (B) The level of hippocampal GSH was detected by glutathione (GSH) enzyme-linked immunosorbent assay (ELISA) kit. (C) The activity of hippocampal SOD was determined by NBT kit. Date are mean ± SEM ( n = 3–4/group). *** P < 0.001, vs. control group; ### P < 0.001, vs. CRS-exposed alone group; &&& P < 0.001, vs. co-treatment with NaHS and CRS group.

Article Snippet: The malondialdehyde (MDA) enzyme-linked immunosorbent assay (ELISA) kit was supplied by Uscn Life Science, Inc. (Wuhan, China).

Techniques: Enzyme-linked Immunosorbent Assay, Activity Assay

Journal: Frontiers in Cardiovascular Medicine

Article Title: Slow flow induces endothelial dysfunction by regulating thioredoxin-interacting protein-mediated oxidative metabolism and vascular inflammation

doi: 10.3389/fcvm.2022.1064375

Figure Lengend Snippet: Plasma TXNIP and MDA were elevated in the coronary of patients with coronary slow flow phenomenon. (A) Plasma TXNIP was measured by using ELISA; (B) Plasma MDA was measured by using ELISA; * P < 0.05 vs. Con, # P < 0.05 vs. SF-CA.

Article Snippet: Plasma TXNIP concentration was measured using human TXNIP and malondialdehyde ELISA kit (MyBioSource), according to the manufacturer’s instructions.

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay